How to Operate the SEM

So now that we know a little background, let's see how to image a sample with the low vacuum SEM.

Before imaging can take place, the SEM needs to be "warmed up" for at least an hour to give the vacuum pumps a chance to heat up and evacuate the column.

When the column is under vacuum, the front panel on the specimen chamber door has the EVAC button lit up.

 

Since the SEM is under vacuum, the column has 
to be vented first before the specimen chamber door
can be opened.
This is done by pressing the VENT button of the chamber door.

 

The previous sample is then removed.

SEM Web Site created and maintained by David J. Wright and Shirley J. Wright
Department of Biology, The University of Dayton.
All images and text are copyright protected.  All rights reserved.
Copyright © 1999 Shirley J. Wright (Shirley.Wright@notes.udayton.edu)